Journal: bioRxiv

Article Title: RGG peptide induces the disassembly of disease-relevant FUS and TDP43 condensates

doi: 10.1101/2025.03.19.643735

Figure Lengend Snippet: (A) Graph representing the distribution of FUS-P525L protein in nucleus and cytoplasm. The fluorescent intensities of the nucleus and cytoplasm were calculated from the experiment as performed in , and the ratio is plotted here. A student-paired t-test was used to calculate the significance value (n=6). (B) Incucyte images (real-time cell death analysis) representing the cellular uptake of propidium iodide (PI) in different conditions in HeLa cells. Scale bar=100um. (C) Graph representing the number of propidium iodide (PI) positive cells (dead cells) from the experiment as performed in B. The time on the x-axis reflects the time-point after transfection in hours. The significance was calculated using 2-way ANOVA with multiple comparisons (n=4). Error bars in all graphs represent mean ±SEM, and the same color points in A depict the data from a single experimental set. *, **, ***, and **** denote p-value≤0.05, ≤0.01, ≤0.001, and ≤0.0001, respectively.

Article Snippet: The cell death analysis was performed using the IncuCyte S3 live-cell analysis instrument (Sartorius), and the change in the number of PI-positive cells (dead cells) in different conditions was plotted in the graph.

Techniques: Transfection

Journal: bioRxiv

Article Title: RGG peptide induces the disassembly of disease-relevant FUS and TDP43 condensates

doi: 10.1101/2025.03.19.643735

Figure Lengend Snippet: Incucyte images representing the cellular uptake of propidium iodide (PI) in different conditions in HeLa cells. Scale bar=100um. The images are part of and .

Article Snippet: The cell death analysis was performed using the IncuCyte S3 live-cell analysis instrument (Sartorius), and the change in the number of PI-positive cells (dead cells) in different conditions was plotted in the graph.

Techniques:

Journal: Cellular and molecular life sciences : CMLS

Article Title: EphA2 promotes the transcription of KLF4 to facilitate stemness in oral squamous cell carcinoma.

doi: 10.1007/s00018-024-05325-w

Figure Lengend Snippet: Fig. 5 EphA2 promotes the nuclear translocation of YAP by activating ERK pathway. A The correlation between YAP and EphA2 acquired by IHC. B Knockdown of EphA2 reduced the expression of YAP. C, D Knockdown of EphA2 reduced the nuclear expression of YAP detected by confocal micrographs (C) and western blotting (D). Scale bars: 20 μm. E Knockdown of EphA2 reduced the pERK protein expression. F, G Total (F) and nuclear (G) expression of YAP were upregulated after adding ERK activator (TBHQ, 5 µM) in shEphA2-OSCC cells. H, I Total (H) and nuclear (I) expression of YAP were decreased after adding ERK inhibitor (SCH772984, 10 µM) in OE- EphA2-OSCC cells

Article Snippet: EphA2 (#66736-1-Ig, 1:5,000), CD133 (#18470-1-AP, 1:2,000), CD44 (#15675-1-AP, 1:2,000), ALDH1A1 (#15910-1-AP, 1:1,000) and YAP (#13584-1-AP, 1:2,000 and #66900- 1-Ig, 1:5,000) antibodies were purchased from Proteintech (Wuhan, China).

Techniques: Translocation Assay, Knockdown, Expressing, Western Blot

Journal: Cellular and molecular life sciences : CMLS

Article Title: EphA2 promotes the transcription of KLF4 to facilitate stemness in oral squamous cell carcinoma.

doi: 10.1007/s00018-024-05325-w

Figure Lengend Snippet: Fig. 7 EphA2 inhibits the growth and stemness of OSCC cells in vivo. A, B The suppression of CSC-related markers expression by knockdown of EphA2 could be reversed by overex pression of YAP (A) or KLF4 (B). C Tumor images of cells pretreated with knockdown of EphA2 or overexpres sion of YAP or overexpression of KLF4 (cell dose = 3 × 106, n = 7). D Growth curves of tumors that measured every 7 days. E-G Representative IHC images of Ki67 and CD44 in excised tumor tissues (E), and their histochem istry score (F, G). Scale bars: 20 μm

Article Snippet: EphA2 (#66736-1-Ig, 1:5,000), CD133 (#18470-1-AP, 1:2,000), CD44 (#15675-1-AP, 1:2,000), ALDH1A1 (#15910-1-AP, 1:1,000) and YAP (#13584-1-AP, 1:2,000 and #66900- 1-Ig, 1:5,000) antibodies were purchased from Proteintech (Wuhan, China).

Techniques: In Vivo, Expressing, Knockdown, Over Expression

Journal: Cellular and molecular life sciences : CMLS

Article Title: EphA2 promotes the transcription of KLF4 to facilitate stemness in oral squamous cell carcinoma.

doi: 10.1007/s00018-024-05325-w

Figure Lengend Snippet: Fig. 8 A schematic model of this study. EphA2 activates the ERK pathway, which leads to nuclear translocation of YAP. YAP subsequently binds to TEAD3 to form a transcriptional complex, which activates the transcrip tion of KLF4, a key transcription factor for CSCs. This ultimately enhances OSCC cell stemness

Article Snippet: EphA2 (#66736-1-Ig, 1:5,000), CD133 (#18470-1-AP, 1:2,000), CD44 (#15675-1-AP, 1:2,000), ALDH1A1 (#15910-1-AP, 1:1,000) and YAP (#13584-1-AP, 1:2,000 and #66900- 1-Ig, 1:5,000) antibodies were purchased from Proteintech (Wuhan, China).

Techniques: Translocation Assay